Western Blot:Article Title: Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Myc, 1:1000 (WB) DSHB Cat#9E10-C Rabbit anti-Myc, 1:500 (IF) Sigma-Aldrich Cat#C3956 Rabbit anti-Myc, 1.5 mg (IP) Millipore Cat#06-549 Mouse anti-HA, 1:500 (IF), 1:1000 (WB), 1.5 mg (IP) BioLegend Cat#901502 Mouse anti-beta tubulin, 1:300 (IF), 1:1000 (WB) DSHB Cat#E7-S Chick anti-beta gal, 1:500 (IF) Abcam Cat#ab9361 Mouse anti-Fasciclin II, 1:50 (IF) DSHB Cat#1D4 Rabbit anti-GFP, 1:300 (IF) Invitrogen Cat#a11122 Rabbit anti-dsRed, 1:200 (IF) Takara Cat#632496 Mouse anti-Scar (supernatant), 1:50 (IF) DSHB Cat#P1C1 Mouse anti-Slit (supernatant), 1:100 (IF) DSHB Cat#C555.6D Mouse anti-Elav (supernatant), 1:20 (IF) DSHB Cat#9F8A9 Mouse anti-Repo (supernatant), 1:20 (IF) DSHB Cat#8D12 Mouse anti-Islet (supernatant), 1:20 (IF) DSHB Cat#40.3A4 Mouse anti-Sex lethal (supernatant), 1:20 (IF) DSHB Cat#M114 Goat anti-Robo3, 1:200 (IHC) R & D systems Cat#AF3076 Mouse anti-Dcc, 1:1000 (WB) BD Biosciences Cat#554223 Mouse anti-Brn-3a, 1:200 (IF) Chemicon Cat#MAB1585 Mouse anti-Lhx1/5, 1:200 (IF) DSHB Cat#4F2 Rabbit anti-Cleaved Caspase-3, 1:1000 (IF) Cell Signaling Cat#9664 Sheep anti-DIG-POD antibody, 1:60 (RNA FISH) Roche Cat#11207733910 Sheep Anti-Digoxigenin-AP, Fab fragments, 1:1000 (RNA FISH) Roche Cat#11093274910 Alexa647 Goat anti-HRP, 1:500 (IF) Jackson Immnuoresearch Cat#123-605-021 Goat anti-Mouse HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#115-035-146 Goat anti-Rabbit HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#111-035-003 Cy3 Donkey anti-Goat, 1:500 (IF) Jackson Immnuoresearch Cat#705-165-147 Alexa488 Goat anti-Rabbit, 1:500 (IF) Invitrogen Cat#A11034 Alexa488 Goat anti-Mouse, 1:500 (IF) Invitrogen Cat#A11029 Alexa488 Goat anti-Chick, 1:500 (IF) Invitrogen Cat#A11039 Cy3 Goat anti-Mouse, 1:500 (IF) Jackson Immnuoresearch Cat#115-165-003 Cy3 Goat anti-Rabbit, 1:500 (IF) Jackson Immnuoresearch Cat#111-165-003 Cy3 Goat anti-Chick, 1:500 (IF) Abcam Cat#ab97145 Chemicals, peptides, and recombinant proteins DMEM Invitrogen Cat#11965084 Horse serum Gibco Cat#16050122 Fetal bovine serum Gibco Cat#10437-028 Normal goat serum Gibco Cat#16210-064 Opti-MEM Gibco Cat#31985-070 F12 Gibco Cat#11765-054 Glucose Thermo Fisher Cat#D16-500 100x Pen/Strep/Glutamine Gibco Cat#10378-016 HBSS Gibco Cat#14175-079 Trypsin Gibco Cat#25300054 Neurobasal Gibco Cat#21103-049 (Continued on next page) e1 Cell Reports 41, 111785, December 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER FBS Gibco Cat#10437-028 B-27 ThermoFisher Cat#A3582801 Effectene Transfection Reagent Qiagen Cat#301425 Direct PCR Lysis Reagent (Mouse Tail) Viagen Biotech Cat#101-T KAPA2G HotStart ReadyMix with dye Roche Cat#KK5609 RNeasy Plus Mini Kit Qiagen Cat#74134 SuperScriptIV Life Technologies Cat#18090200 Fast SYBR Master Mix Life Technologies Cat#4385617 Rat Tail Collagen Corning Cat#354249 Paraformaldehyde 16% Solution, EM grade Electron Microscopy Services Cat#15710 Epredia Aqua-Mount Slide Mounting Media Epredia Cat#13800 Epredia Neg-50 Frozen Section Medium Epredia Cat# 6502 Phorbol 12-myristate 13-acetate, PMA Sigma-Aldrich Cat#P8139 GM6001 Millipore Cat#364205-1MG TAPI-1 Sigma-Aldrich Cat#579053-500UG GI254023X Sigma-Aldrich Cat#SML0789-5MG GM1489 Calbiochem Cat#364200 Formamide (Molecular Biology) Fisher BioReagents Cat#BP227500 Denhardt0s Solution 50x Sigma-Aldrich Cat# D2532 Salmon Sperm DNA Stratagene Cat#201190 tRNA Roche Cat#10109541001 Heparin Sigma-Aldrich Cat#H4784 SSC, 20X Invitrogen Cat#15557036 Dextran Sulfate Fisher BioReagents Cat# BP1585100 Tween-20 Bio-Rad Cat#1706531 Triton X-100 Sigma-Aldrich Cat#T8787-100ML TSA fluorescein system AKOYA Cat#NEL701A001KT Recombinant Mouse Netrin-1 Protein R & D systems Cat#1109-N1-025 Schneider’s Drosophila Medium Life Technologies Cat#21720024 Surfact-AMPS NP40 ThermoFisher Cat#85124 Sodium orthovanadate Sigma-Aldrich Cat#S6508 Protease Inhibitor (Complete) Roche Cat#11697498001 2x Laemmli Sample Buffer Bio-Rad Cat#1610737 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 BME (2-mercaptoethanol) Bio-Rad Cat#1610710 Clarity Western ECL Substrate Bio-Rad Cat#1705061 Essendant Liquid Bleach Essendant Cat#KIKBLEACH6 NotI New England Biolabs Cat#R3189S XbaI New England Biolabs Cat#R0145S EagI New England Biolabs Cat#R3505S XhoI New England Biolabs Cat#R0146S BbsI New England Biolabs Cat#R3539S AseI New England Biolabs Cat#R0526S DpnI New England Biolabs Cat#R0176S T7 RNA Polymerase Promega Corp. Cat#P2075 10x DIG labeled-NTP Roche Cat#11277073910 DNAse Roche Cat#776785 Protein A Agarose beads Invitrogen Cat#15918-014 rProteinG Agarose beads Invitrogen Cat#15920-010 Nitro blue tetrazolium (NBT) Roche Cat#11383213001 (Continued on next page) Cell Reports 41, 111785, December 6, 2022 e2
Immunohistochemistry:Article Title: Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Myc, 1:1000 (WB) DSHB Cat#9E10-C Rabbit anti-Myc, 1:500 (IF) Sigma-Aldrich Cat#C3956 Rabbit anti-Myc, 1.5 mg (IP) Millipore Cat#06-549 Mouse anti-HA, 1:500 (IF), 1:1000 (WB), 1.5 mg (IP) BioLegend Cat#901502 Mouse anti-beta tubulin, 1:300 (IF), 1:1000 (WB) DSHB Cat#E7-S Chick anti-beta gal, 1:500 (IF) Abcam Cat#ab9361 Mouse anti-Fasciclin II, 1:50 (IF) DSHB Cat#1D4 Rabbit anti-GFP, 1:300 (IF) Invitrogen Cat#a11122 Rabbit anti-dsRed, 1:200 (IF) Takara Cat#632496 Mouse anti-Scar (supernatant), 1:50 (IF) DSHB Cat#P1C1 Mouse anti-Slit (supernatant), 1:100 (IF) DSHB Cat#C555.6D Mouse anti-Elav (supernatant), 1:20 (IF) DSHB Cat#9F8A9 Mouse anti-Repo (supernatant), 1:20 (IF) DSHB Cat#8D12 Mouse anti-Islet (supernatant), 1:20 (IF) DSHB Cat#40.3A4 Mouse anti-Sex lethal (supernatant), 1:20 (IF) DSHB Cat#M114 Goat anti-Robo3, 1:200 (IHC) R & D systems Cat#AF3076 Mouse anti-Dcc, 1:1000 (WB) BD Biosciences Cat#554223 Mouse anti-Brn-3a, 1:200 (IF) Chemicon Cat#MAB1585 Mouse anti-Lhx1/5, 1:200 (IF) DSHB Cat#4F2 Rabbit anti-Cleaved Caspase-3, 1:1000 (IF) Cell Signaling Cat#9664 Sheep anti-DIG-POD antibody, 1:60 (RNA FISH) Roche Cat#11207733910 Sheep Anti-Digoxigenin-AP, Fab fragments, 1:1000 (RNA FISH) Roche Cat#11093274910 Alexa647 Goat anti-HRP, 1:500 (IF) Jackson Immnuoresearch Cat#123-605-021 Goat anti-Mouse HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#115-035-146 Goat anti-Rabbit HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#111-035-003 Cy3 Donkey anti-Goat, 1:500 (IF) Jackson Immnuoresearch Cat#705-165-147 Alexa488 Goat anti-Rabbit, 1:500 (IF) Invitrogen Cat#A11034 Alexa488 Goat anti-Mouse, 1:500 (IF) Invitrogen Cat#A11029 Alexa488 Goat anti-Chick, 1:500 (IF) Invitrogen Cat#A11039 Cy3 Goat anti-Mouse, 1:500 (IF) Jackson Immnuoresearch Cat#115-165-003 Cy3 Goat anti-Rabbit, 1:500 (IF) Jackson Immnuoresearch Cat#111-165-003 Cy3 Goat anti-Chick, 1:500 (IF) Abcam Cat#ab97145 Chemicals, peptides, and recombinant proteins DMEM Invitrogen Cat#11965084 Horse serum Gibco Cat#16050122 Fetal bovine serum Gibco Cat#10437-028 Normal goat serum Gibco Cat#16210-064 Opti-MEM Gibco Cat#31985-070 F12 Gibco Cat#11765-054 Glucose Thermo Fisher Cat#D16-500 100x Pen/Strep/Glutamine Gibco Cat#10378-016 HBSS Gibco Cat#14175-079 Trypsin Gibco Cat#25300054 Neurobasal Gibco Cat#21103-049 (Continued on next page) e1 Cell Reports 41, 111785, December 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER FBS Gibco Cat#10437-028 B-27 ThermoFisher Cat#A3582801 Effectene Transfection Reagent Qiagen Cat#301425 Direct PCR Lysis Reagent (Mouse Tail) Viagen Biotech Cat#101-T KAPA2G HotStart ReadyMix with dye Roche Cat#KK5609 RNeasy Plus Mini Kit Qiagen Cat#74134 SuperScriptIV Life Technologies Cat#18090200 Fast SYBR Master Mix Life Technologies Cat#4385617 Rat Tail Collagen Corning Cat#354249 Paraformaldehyde 16% Solution, EM grade Electron Microscopy Services Cat#15710 Epredia Aqua-Mount Slide Mounting Media Epredia Cat#13800 Epredia Neg-50 Frozen Section Medium Epredia Cat# 6502 Phorbol 12-myristate 13-acetate, PMA Sigma-Aldrich Cat#P8139 GM6001 Millipore Cat#364205-1MG TAPI-1 Sigma-Aldrich Cat#579053-500UG GI254023X Sigma-Aldrich Cat#SML0789-5MG GM1489 Calbiochem Cat#364200 Formamide (Molecular Biology) Fisher BioReagents Cat#BP227500 Denhardt0s Solution 50x Sigma-Aldrich Cat# D2532 Salmon Sperm DNA Stratagene Cat#201190 tRNA Roche Cat#10109541001 Heparin Sigma-Aldrich Cat#H4784 SSC, 20X Invitrogen Cat#15557036 Dextran Sulfate Fisher BioReagents Cat# BP1585100 Tween-20 Bio-Rad Cat#1706531 Triton X-100 Sigma-Aldrich Cat#T8787-100ML TSA fluorescein system AKOYA Cat#NEL701A001KT Recombinant Mouse Netrin-1 Protein R & D systems Cat#1109-N1-025 Schneider’s Drosophila Medium Life Technologies Cat#21720024 Surfact-AMPS NP40 ThermoFisher Cat#85124 Sodium orthovanadate Sigma-Aldrich Cat#S6508 Protease Inhibitor (Complete) Roche Cat#11697498001 2x Laemmli Sample Buffer Bio-Rad Cat#1610737 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 BME (2-mercaptoethanol) Bio-Rad Cat#1610710 Clarity Western ECL Substrate Bio-Rad Cat#1705061 Essendant Liquid Bleach Essendant Cat#KIKBLEACH6 NotI New England Biolabs Cat#R3189S XbaI New England Biolabs Cat#R0145S EagI New England Biolabs Cat#R3505S XhoI New England Biolabs Cat#R0146S BbsI New England Biolabs Cat#R3539S AseI New England Biolabs Cat#R0526S DpnI New England Biolabs Cat#R0176S T7 RNA Polymerase Promega Corp. Cat#P2075 10x DIG labeled-NTP Roche Cat#11277073910 DNAse Roche Cat#776785 Protein A Agarose beads Invitrogen Cat#15918-014 rProteinG Agarose beads Invitrogen Cat#15920-010 Nitro blue tetrazolium (NBT) Roche Cat#11383213001 (Continued on next page) Cell Reports 41, 111785, December 6, 2022 e2
Fluorescence In Situ Hybridization:Article Title: Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Myc, 1:1000 (WB) DSHB Cat#9E10-C Rabbit anti-Myc, 1:500 (IF) Sigma-Aldrich Cat#C3956 Rabbit anti-Myc, 1.5 mg (IP) Millipore Cat#06-549 Mouse anti-HA, 1:500 (IF), 1:1000 (WB), 1.5 mg (IP) BioLegend Cat#901502 Mouse anti-beta tubulin, 1:300 (IF), 1:1000 (WB) DSHB Cat#E7-S Chick anti-beta gal, 1:500 (IF) Abcam Cat#ab9361 Mouse anti-Fasciclin II, 1:50 (IF) DSHB Cat#1D4 Rabbit anti-GFP, 1:300 (IF) Invitrogen Cat#a11122 Rabbit anti-dsRed, 1:200 (IF) Takara Cat#632496 Mouse anti-Scar (supernatant), 1:50 (IF) DSHB Cat#P1C1 Mouse anti-Slit (supernatant), 1:100 (IF) DSHB Cat#C555.6D Mouse anti-Elav (supernatant), 1:20 (IF) DSHB Cat#9F8A9 Mouse anti-Repo (supernatant), 1:20 (IF) DSHB Cat#8D12 Mouse anti-Islet (supernatant), 1:20 (IF) DSHB Cat#40.3A4 Mouse anti-Sex lethal (supernatant), 1:20 (IF) DSHB Cat#M114 Goat anti-Robo3, 1:200 (IHC) R & D systems Cat#AF3076 Mouse anti-Dcc, 1:1000 (WB) BD Biosciences Cat#554223 Mouse anti-Brn-3a, 1:200 (IF) Chemicon Cat#MAB1585 Mouse anti-Lhx1/5, 1:200 (IF) DSHB Cat#4F2 Rabbit anti-Cleaved Caspase-3, 1:1000 (IF) Cell Signaling Cat#9664 Sheep anti-DIG-POD antibody, 1:60 (RNA FISH) Roche Cat#11207733910 Sheep Anti-Digoxigenin-AP, Fab fragments, 1:1000 (RNA FISH) Roche Cat#11093274910 Alexa647 Goat anti-HRP, 1:500 (IF) Jackson Immnuoresearch Cat#123-605-021 Goat anti-Mouse HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#115-035-146 Goat anti-Rabbit HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#111-035-003 Cy3 Donkey anti-Goat, 1:500 (IF) Jackson Immnuoresearch Cat#705-165-147 Alexa488 Goat anti-Rabbit, 1:500 (IF) Invitrogen Cat#A11034 Alexa488 Goat anti-Mouse, 1:500 (IF) Invitrogen Cat#A11029 Alexa488 Goat anti-Chick, 1:500 (IF) Invitrogen Cat#A11039 Cy3 Goat anti-Mouse, 1:500 (IF) Jackson Immnuoresearch Cat#115-165-003 Cy3 Goat anti-Rabbit, 1:500 (IF) Jackson Immnuoresearch Cat#111-165-003 Cy3 Goat anti-Chick, 1:500 (IF) Abcam Cat#ab97145 Chemicals, peptides, and recombinant proteins DMEM Invitrogen Cat#11965084 Horse serum Gibco Cat#16050122 Fetal bovine serum Gibco Cat#10437-028 Normal goat serum Gibco Cat#16210-064 Opti-MEM Gibco Cat#31985-070 F12 Gibco Cat#11765-054 Glucose Thermo Fisher Cat#D16-500 100x Pen/Strep/Glutamine Gibco Cat#10378-016 HBSS Gibco Cat#14175-079 Trypsin Gibco Cat#25300054 Neurobasal Gibco Cat#21103-049 (Continued on next page) e1 Cell Reports 41, 111785, December 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER FBS Gibco Cat#10437-028 B-27 ThermoFisher Cat#A3582801 Effectene Transfection Reagent Qiagen Cat#301425 Direct PCR Lysis Reagent (Mouse Tail) Viagen Biotech Cat#101-T KAPA2G HotStart ReadyMix with dye Roche Cat#KK5609 RNeasy Plus Mini Kit Qiagen Cat#74134 SuperScriptIV Life Technologies Cat#18090200 Fast SYBR Master Mix Life Technologies Cat#4385617 Rat Tail Collagen Corning Cat#354249 Paraformaldehyde 16% Solution, EM grade Electron Microscopy Services Cat#15710 Epredia Aqua-Mount Slide Mounting Media Epredia Cat#13800 Epredia Neg-50 Frozen Section Medium Epredia Cat# 6502 Phorbol 12-myristate 13-acetate, PMA Sigma-Aldrich Cat#P8139 GM6001 Millipore Cat#364205-1MG TAPI-1 Sigma-Aldrich Cat#579053-500UG GI254023X Sigma-Aldrich Cat#SML0789-5MG GM1489 Calbiochem Cat#364200 Formamide (Molecular Biology) Fisher BioReagents Cat#BP227500 Denhardt0s Solution 50x Sigma-Aldrich Cat# D2532 Salmon Sperm DNA Stratagene Cat#201190 tRNA Roche Cat#10109541001 Heparin Sigma-Aldrich Cat#H4784 SSC, 20X Invitrogen Cat#15557036 Dextran Sulfate Fisher BioReagents Cat# BP1585100 Tween-20 Bio-Rad Cat#1706531 Triton X-100 Sigma-Aldrich Cat#T8787-100ML TSA fluorescein system AKOYA Cat#NEL701A001KT Recombinant Mouse Netrin-1 Protein R & D systems Cat#1109-N1-025 Schneider’s Drosophila Medium Life Technologies Cat#21720024 Surfact-AMPS NP40 ThermoFisher Cat#85124 Sodium orthovanadate Sigma-Aldrich Cat#S6508 Protease Inhibitor (Complete) Roche Cat#11697498001 2x Laemmli Sample Buffer Bio-Rad Cat#1610737 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 BME (2-mercaptoethanol) Bio-Rad Cat#1610710 Clarity Western ECL Substrate Bio-Rad Cat#1705061 Essendant Liquid Bleach Essendant Cat#KIKBLEACH6 NotI New England Biolabs Cat#R3189S XbaI New England Biolabs Cat#R0145S EagI New England Biolabs Cat#R3505S XhoI New England Biolabs Cat#R0146S BbsI New England Biolabs Cat#R3539S AseI New England Biolabs Cat#R0526S DpnI New England Biolabs Cat#R0176S T7 RNA Polymerase Promega Corp. Cat#P2075 10x DIG labeled-NTP Roche Cat#11277073910 DNAse Roche Cat#776785 Protein A Agarose beads Invitrogen Cat#15918-014 rProteinG Agarose beads Invitrogen Cat#15920-010 Nitro blue tetrazolium (NBT) Roche Cat#11383213001 (Continued on next page) Cell Reports 41, 111785, December 6, 2022 e2
Recombinant:Article Title: Tace/ADAM17 is a bi-directional regulator of axon guidance that coordinates distinct Frazzled and Dcc receptor signaling outputs.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Myc, 1:1000 (WB) DSHB Cat#9E10-C Rabbit anti-Myc, 1:500 (IF) Sigma-Aldrich Cat#C3956 Rabbit anti-Myc, 1.5 mg (IP) Millipore Cat#06-549 Mouse anti-HA, 1:500 (IF), 1:1000 (WB), 1.5 mg (IP) BioLegend Cat#901502 Mouse anti-beta tubulin, 1:300 (IF), 1:1000 (WB) DSHB Cat#E7-S Chick anti-beta gal, 1:500 (IF) Abcam Cat#ab9361 Mouse anti-Fasciclin II, 1:50 (IF) DSHB Cat#1D4 Rabbit anti-GFP, 1:300 (IF) Invitrogen Cat#a11122 Rabbit anti-dsRed, 1:200 (IF) Takara Cat#632496 Mouse anti-Scar (supernatant), 1:50 (IF) DSHB Cat#P1C1 Mouse anti-Slit (supernatant), 1:100 (IF) DSHB Cat#C555.6D Mouse anti-Elav (supernatant), 1:20 (IF) DSHB Cat#9F8A9 Mouse anti-Repo (supernatant), 1:20 (IF) DSHB Cat#8D12 Mouse anti-Islet (supernatant), 1:20 (IF) DSHB Cat#40.3A4 Mouse anti-Sex lethal (supernatant), 1:20 (IF) DSHB Cat#M114 Goat anti-Robo3, 1:200 (IHC) R & D systems Cat#AF3076 Mouse anti-Dcc, 1:1000 (WB) BD Biosciences Cat#554223 Mouse anti-Brn-3a, 1:200 (IF) Chemicon Cat#MAB1585 Mouse anti-Lhx1/5, 1:200 (IF) DSHB Cat#4F2 Rabbit anti-Cleaved Caspase-3, 1:1000 (IF) Cell Signaling Cat#9664 Sheep anti-DIG-POD antibody, 1:60 (RNA FISH) Roche Cat#11207733910 Sheep Anti-Digoxigenin-AP, Fab fragments, 1:1000 (RNA FISH) Roche Cat#11093274910 Alexa647 Goat anti-HRP, 1:500 (IF) Jackson Immnuoresearch Cat#123-605-021 Goat anti-Mouse HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#115-035-146 Goat anti-Rabbit HRP, 1:10,000 (WB) Jackson Immnuoresearch Cat#111-035-003 Cy3 Donkey anti-Goat, 1:500 (IF) Jackson Immnuoresearch Cat#705-165-147 Alexa488 Goat anti-Rabbit, 1:500 (IF) Invitrogen Cat#A11034 Alexa488 Goat anti-Mouse, 1:500 (IF) Invitrogen Cat#A11029 Alexa488 Goat anti-Chick, 1:500 (IF) Invitrogen Cat#A11039 Cy3 Goat anti-Mouse, 1:500 (IF) Jackson Immnuoresearch Cat#115-165-003 Cy3 Goat anti-Rabbit, 1:500 (IF) Jackson Immnuoresearch Cat#111-165-003 Cy3 Goat anti-Chick, 1:500 (IF) Abcam Cat#ab97145 Chemicals, peptides, and recombinant proteins DMEM Invitrogen Cat#11965084 Horse serum Gibco Cat#16050122 Fetal bovine serum Gibco Cat#10437-028 Normal goat serum Gibco Cat#16210-064 Opti-MEM Gibco Cat#31985-070 F12 Gibco Cat#11765-054 Glucose Thermo Fisher Cat#D16-500 100x Pen/Strep/Glutamine Gibco Cat#10378-016 HBSS Gibco Cat#14175-079 Trypsin Gibco Cat#25300054 Neurobasal Gibco Cat#21103-049 (Continued on next page) e1 Cell Reports 41, 111785, December 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER FBS Gibco Cat#10437-028 B-27 ThermoFisher Cat#A3582801 Effectene Transfection Reagent Qiagen Cat#301425 Direct PCR Lysis Reagent (Mouse Tail) Viagen Biotech Cat#101-T KAPA2G HotStart ReadyMix with dye Roche Cat#KK5609 RNeasy Plus Mini Kit Qiagen Cat#74134 SuperScriptIV Life Technologies Cat#18090200 Fast SYBR Master Mix Life Technologies Cat#4385617 Rat Tail Collagen Corning Cat#354249 Paraformaldehyde 16% Solution, EM grade Electron Microscopy Services Cat#15710 Epredia Aqua-Mount Slide Mounting Media Epredia Cat#13800 Epredia Neg-50 Frozen Section Medium Epredia Cat# 6502 Phorbol 12-myristate 13-acetate, PMA Sigma-Aldrich Cat#P8139 GM6001 Millipore Cat#364205-1MG TAPI-1 Sigma-Aldrich Cat#579053-500UG GI254023X Sigma-Aldrich Cat#SML0789-5MG GM1489 Calbiochem Cat#364200 Formamide (Molecular Biology) Fisher BioReagents Cat#BP227500 Denhardt0s Solution 50x Sigma-Aldrich Cat# D2532 Salmon Sperm DNA Stratagene Cat#201190 tRNA Roche Cat#10109541001 Heparin Sigma-Aldrich Cat#H4784 SSC, 20X Invitrogen Cat#15557036 Dextran Sulfate Fisher BioReagents Cat# BP1585100 Tween-20 Bio-Rad Cat#1706531 Triton X-100 Sigma-Aldrich Cat#T8787-100ML TSA fluorescein system AKOYA Cat#NEL701A001KT Recombinant Mouse Netrin-1 Protein R & D systems Cat#1109-N1-025 Schneider’s Drosophila Medium Life Technologies Cat#21720024 Surfact-AMPS NP40 ThermoFisher Cat#85124 Sodium orthovanadate Sigma-Aldrich Cat#S6508 Protease Inhibitor (Complete) Roche Cat#11697498001 2x Laemmli Sample Buffer Bio-Rad Cat#1610737 4x Laemmli Sample Buffer Bio-Rad Cat#1610747 BME (2-mercaptoethanol) Bio-Rad Cat#1610710 Clarity Western ECL Substrate Bio-Rad Cat#1705061 Essendant Liquid Bleach Essendant Cat#KIKBLEACH6 NotI New England Biolabs Cat#R3189S XbaI New England Biolabs Cat#R0145S EagI New England Biolabs Cat#R3505S XhoI New England Biolabs Cat#R0146S BbsI New England Biolabs Cat#R3539S AseI New England Biolabs Cat#R0526S DpnI New England Biolabs Cat#R0176S T7 RNA Polymerase Promega Corp. Cat#P2075 10x DIG labeled-NTP Roche Cat#11277073910 DNAse Roche Cat#776785 Protein A Agarose beads Invitrogen Cat#15918-014 rProteinG Agarose beads Invitrogen Cat#15920-010 Nitro blue tetrazolium (NBT) Roche Cat#11383213001 (Continued on next page) Cell Reports 41, 111785, December 6, 2022 e2
|
Developmental Studies Hybridoma Bank is a verified supplier 
Developmental Studies Hybridoma Bank manufactures this product 
